18th CAHLN Annual Meeting

 « The last frontier in veterinary diagnostic; applied genomic and high throughput sequencing »

It is with great pleasure that we invite you to the 18th Annual Meeting of the Canadian Animal Health Laboratorians Network (CAHLN) to be held in Saint-Hyacinthe at the Faculty of Veterinary Medicine of the Université de Montréal from May 26-29, 2019.

This meeting was initiated as a mean of networking among animal health laboratorians from across Canada and as a showcase for this field of activity for graduate students. It is a forum of knowledge exchange for researchers and those working in diagnostic laboratories and provides an excellent opportunity for students to present their work.

In addition to the scientific program, the CAHLN annual meeting includes a welcome reception, networking sessions, industry exhibits and a special event on Tuesday night.

We invite you to register and submit an abstract for an oral presentation or poster at the meeting.

Please share the invitation with your colleagues. We look forward to seeing you in May!


Dr. Estela Cornaglia
Chair, 18th CAHLN Annual Meeting


CAHLN 2019 and Special Symposium Program

Sunday May 26th, 2019

17:30 – 20:30 CAHLN Evening Reception (includes: Registration, Diner Cocktail, Art Exhibit, Guided tour of laboratories)



3220 Sicotte, Saint-Hyacinthe, Quebec J2S 2M2


CAHLN 18th Annual Meeting

Faculty of Veterinary Medicine

Université de Montréal

3200 Sicotte, Saint-Hyacinthe

Québec (J2S 2M2)

Monday May 27th, 2019

07:30 – 08:15 Registration

08:15 – 08:30 Official Opening and Welcome by (Room 1134) : Marie-Claude Bélanger – Vice-Dean, Clinical Affairs and Professional Training and Estela Cornaglia – Director of the Diagnostic Service.

Applied genomic and Next Generation Sequencing

Moderator: Carl Gagnon (Room 1134)

08:30 – 09:15 Yanyun Huang “Adapting high throughput sequencing in an animal health laboratory – where are we and where to go?”

09:15 – 10:00 Durda Slavic “Pioneering a new technology in accredited veterinary diagnostic laboratory – things to look forward to, or not…”

10:00 – 10:30 Break, posters, exhibits (Room 0135)

Moderator: John Morris Fairbrother (Room 1134)

10:30 – 11:15 Carl Gagnon “Day-to-day challenges in a high throughput sequencing diagnostic laboratory”

11:15 – 12:00 Oliver Lung “High throughput sequencing at Canada’s Only Containment Level 3 HTS Facility: The first 3 years”

12:00 – 13:30 Lunch, posters, exhibits, Meet the Authors in front of their poster between 12h30 and 13h30 (Room 0135)

12:30 – 13:30 CAHLN Business Meeting (open) (Room 1125)

Moderator: Marie Archambault (Room 1134)

13:30 – 14:15 John Fairbrother “Development of a whole genome sequencing approach for identification and antimicrobial resistance of pathogenic Escherichia coli in the veterinary diagnostic laboratory”

14:15 – 15:00 Discussion panel

15:00 – 15:30 Break, posters, exhibits (Room 0135)

Moderator: Chantale Provost (Room 1134)

15:30 – 15:50 Chantale Provost “Genetic diversity preliminary results of swine influenza virus in Quebec, Canada, in 2018”

15:50 – 16:20 Christopher Adams (Ion Torrent scientific presentation), Targeted genotyping by sequencing (tGBS) a flexible and economical solution for multiple high throughput genotyping applications

16:20 – 16:50 Kahlil Lawless (Illumina scientific presentation), Illumina: Advances of microarray and NGS in animal breeding and health.

16:50 Adjourn

17:00 Guided tour of laboratories

17:00 – 20:00 CAHSN Executive Board meeting (by invitation) (Room 1125)


Tuesday May 28th, 2019

CAPV special session CAHLN

Moderator: Maria Spinato (Room 1134)

08:15 – 08:30 Maria Spinato “Using molecular tools to identify previously undetectable causes of ovine abortion – Helicobacter trogontum

08:30 – 08:50 Isabelle St-Pierre, Review of necropsy cases in Québec

08:50 – 09:10 Isabelle St-Pierre, Interesting cases in Québec

09:10 – 09:30 Magaly Bégin-Pépin, PISAQ Abortion in small ruminants

09:30 – 10:00 Julie-Hélène Fairbrother and Sonia Chénier “Postmortem Laboratory FAD Emergency Exercise – First experience”

10:00 – 10:30 Break, posters, exhibits (Room 0135)

10:30 – 10:50 “Provincial Vet. Lab diagnostic reports”

10:50 – 11:20 Marcelo Gottschalk “Diagnosis of Streptococcus suis in an affected farm: harder than expected”

11:20 – 11:40 Younes Chorfi “Measuring deoxynivalenol in animals”

11:40 – 12:00 Younes Chorfi “Selenium levels in Quebec horses”

12:00 – 13:30 Lunch, posters, exhibits, Meet the Authors in front of their poster between 12h30 and 13h30 (Room 0135)

12:00 – 13:30 TSE Meeting (by invitation) (Room 1125)

Student session

Moderator: Julie-Hélène Fairbrother (Room 1134)

13:30 – 13:45 Kaye Quizon “Development and validation of an indirect ELISA for the serological diagnosis of African swine fever”

13:45 – 14:00 Maodong Zhang “Respiratory viruses identified in western Canadian beef cattle by high throughput sequencing and their association with bovine respiratory disease”

14:00 – 14:15 Mahder Teffera “Generation of Capripoxvirus vaccines to differentiate vaccinated animals from unvaccinated animals and validation of a competitive enzyme linked immunosorbent assay to test vaccine efficiency”

14:15 – 14:30 Christian Lalonde “Whole genome sequencing of porcine reproductive and respiratory syndrome virus (PRRSV) from clinical samples”

14:30 – 14:45 Sean Yeo “Detection of foot-and-mouth disease virus in swine meat juice”

14:45 – 15:00 Daniel Gibson “Multiplex real-time PCR assay for three psittacine viruses”

15:00 – 15:30 Break, posters, exhibits (Room 0135)

Moderator: Neil Pople (Room 1134)

15:30 – 16:00 Grant Maxie “Evolution of veterinary lab testing – from the trenches”

16:00 – 16:30 Johnny Callahan (Tetracore scientific presentation), Cutting edge technology: direct field testing using a portable instrument without nucleic acid extraction.

16:30 – 16:45 Michael Mulvey “Building a new AMR surveillance system in Canada: AMRNet”

17:00 Social activity, Bateau Mouche, departure of bus.

18:30 – 22:00 Social activity, Bateau Mouche


Wednesday May 29th, 2019

Plenary session

Moderator: Grant Maxie (Room 1134)

08:10 – 08:40 Anatoliy Trokhymchuk “Canadian Animal Health Technology and Intelligence Networks (CAHTIN) overview”

08:40 – 09:00 David W. Silversides “Labgenvet.ca, a bilingual web-based resource for genetic diseases in domestic animals”

09:00 – 09:20 Rémi Froment “Optimizing the immunocytochemistry technique”

09:20 – 09:40 Musangu Ngeleka “Rapid detection of bovine respiratory bacterial pathogens and associated antimicrobial resistance profiles by whole-metagenome sequencing of clinical samples”

09:40 – 10:00 Musangu Ngeleka “Diagnosis of canine urinary tract infection; laboratory versus in-clinic test accuracy to guide antimicrobial selection”

10:00 – 10:30 Break (Room 0135)

Moderator: Musangu Ngeleka (Room 1134)

10:30 – 10:50 Sonia Lacouture “Distribution of Streptococcus suis (from 2015 to 2018), Actinobacillus pleuropneumoniae and Haemophilus parasuis (from 2018 to April 2019) isolated from diseased pigs in Quebec”

10:50 – 11:10 Darren Boese “An introduction to the Canadian Animal Health Surveillance Network (CAHSN) laboratory support team and the implementation of the African Swine Fever (ASF) proficiency testing program”

11:10 – 11:30 Chantale Provost “Molecular characterization of complete genome of newly emerging avian reovirus variants and novel strains in Quebec, Canada, 2016-2017”

11:30 Presentation of awards:

  • CAHLN Graduate Student Oral/Poster Presentation Awards
  • CAHLN Graduate Student Travel Awards
  • CAHLN Laboratorian of the Year

12:00 Adjourn




Monday May 27th, 2019


C:\Users\zardonm\AppData\Local\Microsoft\Windows\Temporary Internet Files\Content.Word\Yanyun 2018.jpg Yanyun Huang BAgr (Vet Med), MAgr (Vet Path), MSc, PhD, DACVP Director of Diagnostics and Anatomic Pathologist Prairie Diagnostic Services Inc. 52 Campus Drive, Saskatoon, SK S7N 5B4

08:30 – 09:15

“Adapting high throughput sequencing in an animal health laboratory – where are we and where to go”?

Durda Slavic DVM, MSc, PhD Veterinary Bacteriologist Laboratory Services Division. Animal Health Laboratory University of Guelph

09:15 – 10:00

“Pioneering a new technology in accredited veterinary diagnostic laboratory-things to look forward to or not… ”

Carl A. Gagnon DMV, PhD Directeur/Director – CRIPA Laboratoire des maladies infectieuses virales vétérinaires Laboratoire de diagnostic virologique vétérinaire et de diagnostic moléculaire Faculté de médecine vétérinaire, Université de Montréal

10:30 – 11:15

“Day-to-day challenges in a high throughput sequencing diagnostic laboratory”

http://cahln-rctlsa.com/wp-content/uploads/2018/02/oliver-lung.jpg Oliver Lung PhD Research Scientist/Head, Genomics Unit Canadian Food Inspection Agency / Government of Canada National Centre for Foreign Animal Disease

11:15 – 12:00

“High throughput sequencing at Canada’s Only Containment Level 3 HTS Facility: The first 3 years”

C:\Users\zardonm\AppData\Local\Microsoft\Windows\Temporary Internet Files\Content.Outlook\AYWVA60J\DSC_1790x.JPG John Morris Fairbrother DVM, PhD, Director of Reference Laboratory for Escherichia coli Faculté de médecine vétérinaire, Université de Montréal

13:30 – 14:15

“Development of a whole genome sequencing approach for identification and antimicrobial resistance of pathogenic Escherichia coli in the veterinary diagnostic laboratory”


Tuesday May 28th, 2019

cid:BAFF29F2-55B9-465B-824C-061B34E95AC8 Marcelo Gottschalk Professeur/Professor Faculté de médecine vétérinaire/Faculty of Veterinary Medicine University of Montreal

10:50 – 11:20

“Diagnosis of Streptococcus suis in an affected farm: harder than expected”

C:\Users\zardonm\AppData\Local\Microsoft\Windows\Temporary Internet Files\Content.Outlook\AYWVA60J\Younes Chorfi.jpg Younès Chorfi DVM, MSc, PhD Professeur agrégé (Nutrition et productions animales) Département de Biomédecine Vétérinaire Université de Montréal Faculté de Médecine Vétérinaire

11:20 – 11:40

“Measuring deoxynivalenol in animals”

11:40 – 12:00

“Selenium levels in Quebec horses”

Grant Maxie DVM, PhD, Diplomate ACVP Co-Executive Director, Laboratory Services Division, Director, Animal Health Laboratory, University of Guelph

15:30 – 16:00

“Evolution of veterinary lab testing – from the trenches”



Doaa Nouh Université de Montréal An efficacy trial with dietary chlortetracycline against respiratory pasteurellosis in farmed rabbits
Élizabeth Bélanger Uiversity of Sherbrooke Caractérisation d’Escherichia coli entéropathogènes (EPEC) isolées de porcs du Québec par séquençage de nouvelle génération
Gabriel Desmarais Université de Montréal Five years of monitoring pathogenic Escherichia coli virulence and antimicrobial resistance profiles in pigs in Québec
Ghyslaine Vanier Université de Montréal Characterization of avian pathogenic Escherichia coli (APEC) isolates from clinical cases of colibacillosis from poultry in Québec by NGS
Levon Abrahamyan Université de Montréal Optimization of the efficiency of viral infection for two porcine nidoviruses (PRRSV and PEDV) of veterinary importance
Maud de Lagarde University of Montreal Emergence récente d’un clone ETEC : F4 non-susceptible à l’enrofloxacine dans la population de E. coli pathogènes chez le porc au Québec.
Passoret Vounba Université de Montréal Spread of Escherichia coli carrying blaCTX-M and blaCMY-2 in Senegalese chicken farms through clones and plasmids
Veronique Bournival Université de Montréal Quebec IBV strains follow-up by S1 gene sequencing
Yaima Burgher Université de Montréal Ureaplasma diversum, a bovine pathogen identified for the first time in the respiratory tract of swine in Canada
From Montreal Airport to Saint-Hyacinthe (2 options): Rent a carhttps://www.admtl.com/en/access/car-rental Taxi: https://www.admtl.com/en/access/taxis-limousines or call  514-394-7377 (select the language, than chose option  #2, than #3, #1) Note: it normally starts at 140CAD for a maximum of 4 people (one way). For more information, visit the website.



Hotels Method of Reservation


Additional info
Le Dauphin Saint-Hyacinthe 1250, Daniel-Johnson West Saint-Hyacinthe, Qc, J2S 7K7 Website: https://www.hotelsdauphin.ca/en/hotels/saint-hyacinthe T : 450-774-4418 or 1-800-465-4842 Group code to mention : CAHLN Cut-off date : April 26, 2019

144$ + tx

In single or double occupancy

Includes continental breakfast Computer and free WiFi in each room 3.5Km to FMV (10 min drive)
Holiday Inn Express & Suites 1500 Daniel-Johnson East Saint-Hyacinthe, Qc, J2S 8W5 Website: https://www.ihg.com/holidayinnexpress/hotels/us/en/saint-hyacinthe/yulsh/hoteldetail T: 450-251-1111 Group code to mention: CAHLN Booking link : Canadian Animal Health Laboratorians Network Cut-off date: April 28, 2019

149$ + tx

(Std room)

169$ + tx


Includes buffet-style breakfast Indoor pool Free high-speed internet in all room 4.5Km to FMV (12 min drive)
Sheraton Saint-Hyacinthe Hotel 1315 Daniel-Johnson West Saint-Hyacinthe, Qc, J2S 8S4 Website: https://www.marriott.com/hotels/travel/yulhy-sheraton-saint-hyacinthe-hotel/ T: 1-877-619-0410 Group code to mention: CAHLN Cut-off date: April 24, 2019

155$ + tx

(Std room)

205$ + tx

(Jnr suite)

In single or double occupancy

No breakfast included Indoor pool Free high-speed internet in all room 3.4km to FMV (10 min drive)


Tuesday night 

Come live a typical Montreal experience that will make you discover the city from the Saint- Lawrence River. A shuttle will await the participants at the end of the conferences and will bring them directly to the Old Port of Montreal. Boarding on the Bateau-Mouche will take place at 6:30 pm from Quai Jacques-Cartier. Total duration of the cruise is 3 hours ½, and will end at 10:30 pm. When you return, the shuttle will drop you off at the Faculty of Veterinary Medicine or at one of the 3 designated convention hotels (see Accommodation section). The cruise includes a 4-course dinner, with 3 menu choices to be determined on site, musical entertainment and an immersive sound and light experience, designed by the Quebec company XYZ technologies. Sail the river and the Boucherville Islands. Discover the city and its main attractions by day, but also by night, including the Jacques-Cartier Bridge fully illuminated. The total value of this package is 120$, but is offered at only 80$ for the CAHLN attendees. The deadline to register is April 26, 2019 (see registration section to make the reservation). For more information, visit: https://www.bateaumouche.ca/en/home.html


Extra Activities

Tourism Saint-Hyacinthe

Abstract guidelines

Abstracts must be received by April 26, 2019 Click Here to upload and submit your abstract. Please note that you must also complete, submit and include payment on the registration form to attend the conference. For any questions or concerns, please email : cahlnrctlsa@medvet.umontreal.ca When preparing your abstract, please use the following format instructions:

  • Word Document
  • Arial (11 pt)
  • Title of Abstract in bold
  • Name(s) and affiliation(s) of author(s). Type the first and last name of each author. Follow each author with a numbered affiliation. I.E. (1), (2), etc…
  • Text of Abstract : 400 words or less



Title: Diagnosis of swine respiratory infectious agents using a nano-volume quantitative PCR platform. Author(s) name(s) / Affiliation(s): Philippe Garneau1, Véronique Allard2, Tony Sabetti3, Donald Tremblay2, Carl A. Gagnon1, Marie Archambault1, Josée Harel1. 1Centre de Recherche en Infectiologie Porcine, Faculté de médecine vétérinaire, Université de Montréal; 2Service de diagnostic moléculaire, Faculté de médecine vétérinaire, Université de Montréal; 3NextGen QPCR Systems and Applications, Life Technologies. Abstract: We are developing a novel diagnostic approach for simultaneous identification and quantification of swine respiratory infectious agents bringing losses to the industry. The Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in particular cause important economical losses to swine producers. The virulence of PRRSV is associated with multiple factors including other viral pathogens such as Circovirus, Influenza virus and bacterial infections. The main goal of this project is to develop a comprehensive and efficient identification and characterization diagnostic approach for viral and bacterial agents responsible for swine morbidity and mortality. We propose to use the OpenArray® technology from Life Technologies. This platform, based on nanofluidic q-PCR technology, allows the identification of up to several hundred agents simultaneously. We have reached the first milestone of our study. We selected the target respiratory infectious agents as well as identified representative sequences. Using bioinformatic analysis, we designed DNA primers/probes compatible with the OpenArray® q-PCR technology operating parameters. The specificity and sensitivity parameters characteristic of these sets were evaluated using previously characterized samples. When their real time qPCR results met the platform’s efficiency requirements, the primer/probe sets were kept, otherwise new sets were designed. The first quantification laboratory tests on the sets were conclusive. The capabilities and performance characteristics of the system are being evaluated. The development of rapid and accurate tools for diagnosis is important and contributes to improve animal infectious disease eradication or control programs. It is our hope it will contribute to support a high productivity program of the swine industry.


Poster guidelines

Each author will have a useable area measuring 8 feet (243.84cm) wide x 4 feet (121.92 cm) high.



Graduate Student Travel Awards

The travel awards are intended to help defray the cost of travel and lodging for students traveling to the Annual CAHLN meeting from sites distant to the host site, encouraging student excellence in their laboratory discipline, developing presentation skills (verbal, written, organizational), and promoting networking of students with veterinary laboratorians. This year, funds are available for 2 travel awards of $750 each. Trainees in a Master’s, PhD or residency program enrolled in any discipline in veterinary laboratory medicine including epidemiology, immunology, microbiology (bacteriology, food safety, mycology and virology), molecular diagnostics, parasitology, pathology (anatomic and clinical), public health, and toxicology are encouraged to apply.

The scientific committee has selected the winners of the Graduate Student Travel Awards :

Maodong Zhang

University of Saskatchewan

Daniel Gibson

University of Guelph



 CAHLN Graduate Student Presentation Award

A plaque is awarded annually to a graduate student based on the quality of their presentation at the CAHLN annual meeting.  For this year there will be one oral presentation award and one poster presentation award. In addition to the plaque, a cash prize of 400$ sponsored by the CRIPA will be given to each winner.Presentations are judged on the originality of the subject, contribution of the presentation to our knowledge base, the student’s understanding and delivery of the topic, and ability to answer questions.


Laboratorian of the Year

Who can be nominated: Anyone who has made a significant contribution to veterinary diagnostic laboratory in Canada. A nominee can be an outstanding diagnostician, educator, researcher or mentor of future laboratorians. How to nominate: Send a brief letter of support outlining the nominee’s accomplishments, along with the nominee’s CV, to the Awards Committee of CAHLN, by sending an e-mail to: Estela Cornaglia at estela.cornaglia@umontreal.ca or at cahlnrctlsa@medvet.umontreal.ca, before April 26, 2019. Please write “Laboratorian of the Year” as the subject title.

The Canadian Animal Health Laboratorians Network (CAHLN) is pleased to invite you to be a sponsor of the upcoming national annual meeting on May 26-29, 2019 at the Faculty of Medicine Veterinary. The following Sponsorship Packages detail the benefits and features provided to sponsors of the 18th CAHLN Annual Meeting.

CAHLN exhibit / booth map

The Organizing Committee


Estela Maria Cornaglia

Chair, 18th CAHLN Annual Meeting


Véronique Allard

Véronique Boyer

Guy Fontaine

Maria Zardon

Louise Bazin

Nadine Messier



Scientific committee

Estela Maria Cornaglia DMV, PhD

Carl Gagnon DMV, PhD

Julie-Hélène Fairbrother DMV, MSc, DACVM

Olivia Labrecque DMV, MSc, DACVM


Thanks To Our Sponsors

Gold Sponsor

Tetracore UdM

Silver Sponsors

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Bronze Sponsors

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Other Supporters